14^th International Conference on Biofuels and Bioenergy June 22-23, 2020 | Paris, France

Biography:

Dr. Monika Prakash Rai has completed her Ph.D. from IIT- BHU Varanasi, India jointly with Johannes Gutenberg University, Mainz, Germany in the year 2005. She is Associate Professor at Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India. She has expertise in microbial culture systems and synthesis of value added products. Her major focus area is algal biodiesel, bioremediation, waste utilization, enzymes production, and synthesis of other industrially important products. Her work also emphasizes on the production of green materials from microbial origin and application in the area of nano-biotechnology, corrosion inhibition and antimicrobial activity. She has published more than 25 papers in reputed journals and published many book chapters.

Abstract:

Microalgae have potential to convert CO₂ into carbon skeleton biomass that stores mainly starch and lipid rich compounds, which can be processed for sustainable biofuel production. Although, the high culture cost and low lipid productivity are foremost hurdles for its commercial feasibility in biodiesel application. Hence, these challenges are trying to overcome by establishing mixotrophic mode of cultivation using waste organic carbon sources as low cost substrates. In the present work, the molasses was collected from a sugar refinery and microalgae were isolated from sugar molasses by successive plating on BG-11 supplemented with 1% agar. The microalga isolated was identified as Chlorella sorokiniana (MS) by using 18 S rDNA marker.

Spent wash, a waste effluent of sugar distillery was collected from the same site and characterized for physico-chemical properties including chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP) and total carbon (TC). Cultivation of microalgae was optimized in spent wash media by varying carbon concentration, nitrogen, pH, light intensity and photoperiod using response surface method (RSM). Maximum microalgae biomass of 3g/L was obtained by using 289.6 ml/L of spent wash maintaining at pH 6.5. Fluorescence microscopy confirms the increase in neutral lipids in the cell. Lipid was extracted by solvent extraction method and transesterified to obtain Fatty acid methyl esters (FAME) that was analysed by GC-MS. The FAME profile obtained includes the compounds like palmitic acid, oleic acid, linoleic acid, linolenic acid in a requisite ratio, those are essential for biodiesel synthesis. This study highlights the recycle of sugar distillery waste material spent wash as a nutrient source for microalgae biomass and lipid production for its potential application in biodiesel.

Biography:

Dr. Monika Prakash Rai has completed her Ph.D. from IIT- BHU Varanasi, India jointly with Johannes Gutenberg University, Mainz, Germany in the year 2005. She is Associate Professor at Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India. She has expertise in microbial culture systems and synthesis of value added products. Her major focus area is algal biodiesel, bioremediation, waste utilization, enzymes production, and synthesis of other industrially important products. Her work also emphasizes on the production of green materials from microbial origin and application in the area of nano-biotechnology, corrosion inhibition and antimicrobial activity. She has published more than 25 papers in reputed journals and published many book chapters.

Abstract:

Microalgae have potential to convert CO₂ into carbon skeleton biomass that stores mainly starch and lipid rich compounds, which can be processed for sustainable biofuel production. Although, the high culture cost and low lipid productivity are foremost hurdles for its commercial feasibility in biodiesel application. Hence, these challenges are trying to overcome by establishing mixotrophic mode of cultivation using waste organic carbon sources as low cost substrates. In the present work, the molasses was collected from a sugar refinery and microalgae were isolated from sugar molasses by successive plating on BG-11 supplemented with 1% agar. The microalga isolated was identified as Chlorella sorokiniana (MS) by using 18 S rDNA marker.

Spent wash, a waste effluent of sugar distillery was collected from the same site and characterized for physico-chemical properties including chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP) and total carbon (TC). Cultivation of microalgae was optimized in spent wash media by varying carbon concentration, nitrogen, pH, light intensity and photoperiod using response surface method (RSM). Maximum microalgae biomass of 3g/L was obtained by using 289.6 ml/L of spent wash maintaining at pH 6.5. Fluorescence microscopy confirms the increase in neutral lipids in the cell. Lipid was extracted by solvent extraction method and transesterified to obtain Fatty acid methyl esters (FAME) that was analysed by GC-MS. The FAME profile obtained includes the compounds like palmitic acid, oleic acid, linoleic acid, linolenic acid in a requisite ratio, those are essential for biodiesel synthesis. This study highlights the recycle of sugar distillery waste material spent wash as a nutrient source for microalgae biomass and lipid production for its potential application in biodiesel.

Biography:

Dr. Monika Prakash Rai has completed her Ph.D. from IIT- BHU Varanasi, India jointly with Johannes Gutenberg University, Mainz, Germany in the year 2005. She is Associate Professor at Amity Institute of Biotechnology, Amity University Uttar Pradesh, Noida, India. She has expertise in microbial culture systems and synthesis of value added products. Her major focus area is algal biodiesel, bioremediation, waste utilization, enzymes production, and synthesis of other industrially important products. Her work also emphasizes on the production of green materials from microbial origin and application in the area of nano-biotechnology, corrosion inhibition and antimicrobial activity. She has published more than 25 papers in reputed journals and published many book chapters.

Abstract:

Microalgae have potential to convert CO₂ into carbon skeleton biomass that stores mainly starch and lipid rich compounds, which can be processed for sustainable biofuel production. Although, the high culture cost and low lipid productivity are foremost hurdles for its commercial feasibility in biodiesel application. Hence, these challenges are trying to overcome by establishing mixotrophic mode of cultivation using waste organic carbon sources as low cost substrates. In the present work, the molasses was collected from a sugar refinery and microalgae were isolated from sugar molasses by successive plating on BG-11 supplemented with 1% agar. The microalga isolated was identified as Chlorella sorokiniana (MS) by using 18 S rDNA marker.

Spent wash, a waste effluent of sugar distillery was collected from the same site and characterized for physico-chemical properties including chemical oxygen demand (COD), total nitrogen (TN), total phosphorus (TP) and total carbon (TC). Cultivation of microalgae was optimized in spent wash media by varying carbon concentration, nitrogen, pH, light intensity and photoperiod using response surface method (RSM). Maximum microalgae biomass of 3g/L was obtained by using 289.6 ml/L of spent wash maintaining at pH 6.5. Fluorescence microscopy confirms the increase in neutral lipids in the cell. Lipid was extracted by solvent extraction method and transesterified to obtain Fatty acid methyl esters (FAME) that was analysed by GC-MS. The FAME profile obtained includes the compounds like palmitic acid, oleic acid, linoleic acid, linolenic acid in a requisite ratio, those are essential for biodiesel synthesis. This study highlights the recycle of sugar distillery waste material spent wash as a nutrient source for microalgae biomass and lipid production for its potential application in biodiesel.

Biography:

Iesham alzoubi has completed his PhD at the age of 27 years fromDoctor of Philosophy Degree in Agricultural Mechanization Engineering (University of Tehran – Iran) University and Postdoctoral Studies from School of Surveying Geospatial Engineering-Department of Surveying and Geomatics Engineering, University of Tehran .  Current Job: General commission for scientific Agricultural Research – Damascus – Syria (Mechanical Engineer)  He has published more than 16 papers in reputed journals and has been serving as an editorial board member of repute.

Abstract:

Land leveling is one of the most important steps in soil preparation for agricultural and other purposes. . New techniques based on artificial intelligence, such as Artificial Neural Network, integrating Artificial Neural Network and Imperialist Competitive Algorithm (ICA-ANN), or Genetic Algorithms (GA-ANN), or Particle Swarm Optimization (PSO-ANN) have been employed for developing predictive models to estimate the energy related parameters and the results were compared to SPSS and Sensitivity Analysis  results. In this study, several soil properties such as cut/fill volume, compressibility factor, specific gravity, moisture content, slope of the area, sand percent, and swelling index were measured and their effects on energy consumption were investigated. Totally 90 samples were collected from 3 land areas by grid size of 20m×20m. The aim of this work was to develop predictive models based on artificial intelligence techniques to predict the environmental indicators of land leveling . Results of sensitivity analysis illustrated that only three parameters consist of soil density, soil compressibility, and soil cut/fill volume had meaningful effects on energy consumption.  Among the proposed methods, the GA-ANN had the most capability in prediction of the environmental energy parameters. However, for prediction of LE and FE the ANN and ICA-ANN algorithms had better performance

 .  On the other hand, SPSS software had higher R 2 value than Minitab software and sensitivity analysis and in fact close to the ANN values. Keywords: Energy; Imperialist competitive algorithm; Sensitivity analysis; ANN; Land levelling; Environmental indicators.

Keywords:Artificial Neural Network; energy; environmental research; Imperialist Competitive Algorithm; Sensitivity Analysis

.Originality/value – A limited number of research studies related to energy consumption in land leveling have been done on energy as a function of volume of excavation and embankment. However, in this research, energy and cost of land leveling are shown to be functions of all the properties of the land, including the slope, coefficient of swelling, density of the soil, soil moisture and special weight dirt.

Biography:

Cornelius Claeys is a Brussels-based analyst responsible for European biofuel policies and market developments. He has regularly spoken at biofuels conferences in Europe (overview

available upon demand). Before joining Stratas Advisors, Cornelius worked in Saudi Arabia, where he conducted research on the economic effects of legislative reforms in the energy sector. He also has experience in the European Commission’s DG Trade, Brussels-based think tanks and international business media. Cornelius has a Degree in Applied Economics: Business Engineering and a Master in European Studies from the University of Leuven. He is fluent in Dutch, English, French and Spanish.

Abstract:

This presentation will leverage Stratas Advisors modelling capabilities and experience in regulatory market analysis to provide an outlook of biofuel supply/demand in Europe. Country and fuel specific breakdowns are included, with special focus on volumetric FAME, HVO, ethanol, biomethane and biojet demand forecasts, as well as the implications for regional production capacity. First, an overview of regulatory biofuel demand drivers is provided, including the European Green Deal and its implications for RED II, the Fuel Quality Directive, national blending mandates and global initiatives like CORSIA. The second part zooms in on some of the underlying implications for base-pool demand, cost dimensions and feedstock supply. A third part discusses how road, marine and aviation biofuel markets interrelate to each other. Finally, a detailed quantitative outlook per biofuel is discussed, zooming in on some of the larger European countries before coming to the aggregated EU picture. Stratas Advisors is a global energy research company with offices in Houston, Brussels, Singapore and New Delhi. Our biofuels team is composed of economists, engineers and political scientists, who work together to provide a quarterly biofuels outlook for more than 100 countries worldwide.

Biography:

Mohamed benaddou  is a third year doctoral student. He is working on fungal and enzymatic pretreatment of lignocellulosic biomass as a doctoral project. besides research, he is a qualifying high school teacher since 2009. He obtained his mater in 2012 under the title "mastere of environmental sciences". He participates in several congresses and scientific conferences in Morocco.

Abstract:

In the region of Meknes, Morocco, there is a large amount of lignocellulosic mass rich in cellulose and hemicelluloses but released in the trash. The direct use of this mass to produce bioethanol has a low yield because the cellulose and hemicellulose are protected by lignin hence the need for pretreatment to degrade or modify the lignin to release cellulose and hemicellulose. Biological treatment, especially with fungi, is a very simple method, respects the environment and specifically degrades lignin. The main objective of this project is the evaluation of the impact of the treatment with fungi on the digestibility, lignin content and cellulose crystallinity index of wheat straw, reddish wood sawdust and olive pomace. 

In this study, three fungi, Fusarium oxysporum , Fusarium solani and Fusarium sp were used to treat the lignocellulosic biomass. Samples obtained after 8 weeks of incubation were assayed for digestibility, lignin, and cellulose contents according to the Van Soest method and cellulose cristallinity cellulose. The digestibility, lignin content and cellulose crystallinity index were significantly affected by substrate and treatment. But cellulose did not change among substrates and treatments. The digestibility was higher (p<0.05) in the control than in the three fungi types, and higher (p<0.01) in wheat straw than in the two other substrates.  

Biography:

Dr. V. Himabindu has completed her PhD in 1998 from JNTUH University Hyderabad, Telangana India. She is currently Head and Professor at Centre of Environment, JNTUH. She has published more than 80 papers in reputed International journals and has been serving as an editorial board member of several reputed journals. Till now she has supervised more than 18 PhD students. She has received research grants for more than 20 research proposals from different funding agencies like DBT, DST, MNRE, BARC, MHRD etc. Area of research are Environment and Energy, Waste recovery and reuse, Bioremediation , Biofuels, Carbon nano materials, etc.

Abstract:

Concern about global warming and energy security has led to increased biomass utilization as an alternative feedstock to fossil fuels. Microalgae biofuels are likely to have a much lower impact on the environment. Microalgae cultivation using sewage with industrial flue gases is a promising concept for integrated bio-oil production, CO₂ sequestration and nutrient recovery. Mixotrophic cultivation has given best results for microalgae biomass. Several mechanical and chemical processes are available for extraction of lipids components from microalgae biomass. In organic solvent extraction methods, a prior drying of biomass and recovery of the solvent is required which are energy-intensive. Thus, hydrothermal process overcomes the drawbacks of conventional methods where the biomass is converted into oily components by processing in a hot, pressurized water environment. In this process, in addition to the lipid fraction of microalgae, other value added products such as proteins, carbohydrates and nutrients can also be recovered. In the present study (Scenedesmus quadricauda) was cultivated mixotrophically using sewage wastewater and industrial flue gas in batch and continuous mode. The harvested algae biomass from S. quadricauda was used for the recovery of lipids and bio-oil. The lipids were extracted from the algal biomass using sonication as a cell disruption method followed by solvent (Hexane) extraction and the lipid yield obtained was 8.3 wt%. Hydrothermal process was also carried out for extraction of bio oil and the yield obtained was 18wt%. Nutrients such as NO₃¯ (68%) and PO₄¯ (15%) were also recovered along with bio-oil in hydrothermal process.